DNA

Part:BBa_K4610000

Designed by: Sophie Geierstanger   Group: iGEM23_CCA-San-Diego   (2023-10-07)


Afu8g00520 (Fma-TC)

Afu8g00520 (BBa_K4610000) is a segment of DNA that encodes for the integral membrane protein Fumagillin beta-trans-bergamotene synthase af520/Fumagillan biosynthesis terpene cyclase (Fma-TC).

Fma-TC is used as the first step of the Fumagillan biosynthesis pathway chosen by the CCA-iGEM team. This fumagillin biosynthesis pathway summarized by Guruceaga et. al 2020, consists of two branches that combine the two components required for the generation of fumagilin, a structure composed of a rearranged highly oxygenated sesquiterpene and a polyketide-derived tetraenoic diacid, by esterification. The first branch produces fumagillol and involves Fma-TC and subsequently Afu8g00520. The pathway begins with the initial substrate, farnesyl pyrophosphate (FPP). FPP is then converted to β-trans-bergamotene by Fma-TC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 386
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 386
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 386
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 127


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Categories
Parameters
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